{"id":144,"date":"2021-08-03T15:56:58","date_gmt":"2021-08-03T07:56:58","guid":{"rendered":"http:\/\/phosphoramidite.cn\/en\/?p=144"},"modified":"2021-08-03T15:57:44","modified_gmt":"2021-08-03T07:57:44","slug":"synthesis-of-phosphate-branched-oligonucleotides","status":"publish","type":"post","link":"https:\/\/nugene.com.cn\/en\/index.php\/synthesis-of-phosphate-branched-oligonucleotides\/","title":{"rendered":"Synthesis of Phosphate-Branched Oligonucleotides"},"content":{"rendered":"\n

A solid-phase synthesis for phosphate-branched oligonucleotides is described. The method is based on coupling of a single nucleoside phosphorodiamidite to terminal hydroxyl functions of two solidsupported oligonucleotides. After oxidation of the phosphite triester obtained to a phosphate triester, the third branch is assembled by conventional phosphoramidite chemistry.<\/p>\n\n\n\n

\"\"
Key Steps in Branching of Oligonucleotides Using Phosphorodialkylamidites as Building Blocks<\/figcaption><\/figure>\n\n\n\n

Phosphorodialkylamidites 1-3 may in principle be used in two different ways to obtain phosphate-branched oligonucleotides. Either two solid-supported oligonucleotides are reacted through their terminal hydroxyl functions with a single phosphorodialkylamidite monomer (Reaction A in Scheme 1), or an internucleosidic phosphoramidite linkage of a solid-supported oligonucleotide is converted to a phosphite triester by nucleophilic attack of a monomeric nucleoside hydroxyl function (Reaction B in Scheme 1). The rest of the branches are then assembled, after oxidation of the phosphite triester to a
phosphate ester, by conventional phosphoramidite chemistry. As mentioned above, five different nucleoside phoshorodialkylamidite building blocks (1-3) were prepared, and the coupling reactions indicated in Scheme 1 were studied.<\/p>\n","protected":false},"excerpt":{"rendered":"

A solid-phase synthesis for phosphate-branched oligonucleotides is described. The method is based on coupling of a single nucleoside phosphorodiamidite to terminal hydroxyl functions of two solidsupported oligonucleotides. After oxidation of the phosphite triester obtained to a phosphate triester, the third branch is assembled by conventional phosphoramidite chemistry. Phosphorodialkylamidites 1-3 may in principle be used in …<\/p>\n

Synthesis of Phosphate-Branched Oligonucleotides<\/span> Read More »<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","footnotes":""},"categories":[6,9],"tags":[12],"class_list":["post-144","post","type-post","status-publish","format-standard","hentry","category-news","category-rd","tag-synthesis-of-oligonucleotides"],"yoast_head":"\nSynthesis of Phosphate-Branched Oligonucleotides - Nucleic acid Building Block<\/title>\n<meta name=\"description\" content=\"Nucleic acid Building Block Synthesis of Phosphate-Branched Oligonucleotides\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/nugene.com.cn\/en\/index.php\/synthesis-of-phosphate-branched-oligonucleotides\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Synthesis of Phosphate-Branched Oligonucleotides - 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