Modification of RNA, modification of mRNA, requires the use of modified nucleotides as key starting materials. Nugene Biopharm Science supplies modified nucleotides with high purity, high activity and high transcription.
Pseudo UTP and N1-Methyl Pseudo UTP stock enough for your commerical production. The GMP workshop provide materials for 8 Billion dose mRNA requirement.
| Symbol | Common Name | CAS NO. | Chemical Structure |
| m1A | N1-Methyl ATP |  | |
| m6A | N6-Methyl ATP |  | |
| m5C | 5-Methyl CTP |  | |
| ac4C | N4-Acetyl CTP |  | |
| hm5C | 5-Hydroxymethyl CTP |  | |
| m7G | N7-Methyl GTP |  | |
| s2U | 2-Thio-UTP |  | |
| Ψ | Pseudo UTP | 1175-34-4(free acid) |  |
| m1Ψ | N1-Methyl Pseudo UTP | 1428903-59-6 (free acid) |  |
| mo1Ψ | N1-Methoxy Pseudo UTP |  | |
| m5U | 5-Methoxy UTP |  |
For the above nucleotide materials, Nugene Biopharm Science provides 100mM aqueous solution and lyophilized powder. The aqueous solution is convenient for direct use in research and development, and the lyophilized powder is convenient for transportation by air/ by express, customer laboratory storage, and customer production storage.
RNA, mRNA, and other Nucleic Acid prepared by using modified nucleotides, although the proportion of modified nucleotides is relatively small, often lower than the proportion of natural nucleotides, but usually make RNA, mRNA have better stability and higher Expression efficiency, less toxicity, higher safety.
The use of mRNA produced by modified nucleotides can mimic the recognition of pattern recognition receptors and improve the ability of mRNA to produce protein antigens, thereby stimulating a beneficial immune response and reducing the dose of mRNA used. Both Moderna and BioNtech (Pfizer) have marketed mRNA new Covid-19 vaccines that use modified nucleotides to produce mRNA.
These materils can also be used for synthetic biology research, development and production of in vitro detection test reagents.